Fabrication and Characterization of Glucosamine Hydrochloride from Chitin of Horseshoe Crab Shell (Tachypleus gigas)

Preparation of glucosamine hydrochloride from the chitin horseshoe crab shells using chemical hydrolysis method has been done HCl concentration variation ratios 7%, 9%, 11%,14 % with a ratio 9:1 (v/w) for 4 hours at temperature 90ºC. Determination characteristics was characterized Fourier transform-infra red (FT-IR) spectroscopy, in which obtained OH group were 3446 cm-1 (s), 3448 3450 respectively. For NH 1557 1559 1556 1560 CN 1379 (m), While glycoside bond 1073 (w), 1074 Ultraviolet Spectrophotometer analysis maximum wavelength 197 nm standard solution N-acetyl phosphate acid 0.005%, 7% = 33.67 ppm, 9% 36.35 11% 40.16 14% 43.97 ppm.